The all-premiership clash between Asante Kotoko and Heart of Lions will kick off the FA Cup quarter-final pairings on May 25.
Both sides will meet at the Baba Yara Stadium in one of the two midweek quarter-final matches announced by the organisers of the Cup competition.
The game in Kumasi kicks off at 18:15 GMT [6.15pm].
The other fixture would see Division One campaigners, D'International and FC Nania play at the Tema Stadium in a 15:00 GMT [3.00pm] kick off match.
Berekum Chelsea's chase for their double win of the season will be tested when they travel to the Bolga Park to play Bolga All Stars on June 1 in a 15:00 GMT [3.00pm] kick off.
Also on the same day, the other quarter-final match would see Wa All Stars host Medeama to the Wa Park.
Wednesday, May 25
Asante Kotoko vrs Heart of Lions
D'International vrs FC Nania
Wednesday, June 1
Wa All Stars vrs Medeama SC
Bolga All stars vrs Berekum Chelsea