League organisers, the Premier League Board is demanding ambulance services at every league centre in the Glo Premier League.
After watching health issues at league grounds around the world turn fatal, the PLB says home clubs are to provide the services, which are mandatory.
"The Premier League Board has noted with concern the failure of some clubs to provide ambulance services during their home matches.
"The PLB wishes to stress that this failure is in serious breach of Article 42(1) of the FA Regulations," said the PLB statement.
"It is the PLB’s view in the light of some tragic health related concerns in some football matches, even at the world stage, it is imperative all clubs comply with the directive.
"Accordingly all clubs are directed to provide ambulance services, effective this weekend, to avoid sanctions.
The PLB has also demanded of match co-ordinators and commissioners in the league to ensure that such facilities are available at every match venue.
"By copies of this circular, all General Co-ordinators and Match Commissioners are to ensure that no match under their charge is allowed to commence until an ambulance has been provided."