Ghana's impressive run has been ended by Japan as the Black Stars lose 3-4 in an international friendly.

Black Stars coach Milovan Rajevac made changes to his starting team that qualified Ghana to the 2010 World Cup in Wednesday’s friendly at the Stadion Galgenwaard in Utrecht.

Isaac Vorsah started in central defence alongside John Mensah as Laryea Kingston, Haminu Draman and Asamoah Gyan all substitutes in the World Cup qualifier last Sunday were handed starting roles.

And after 30 minutes, Asamoah Gyan fired Ghana into the lead from a spot kick as the Black Stars held onto their slim lead to end the half.

Back from recess, the Rennes striker doubled Ghana's lead scoring after 46 minutes.

Three minutes later, Kengo Nakumura pulled one back for Japan.

Rajevac made a host changes bringing on goalkeeper William Amamoo for Richard Kingson.

Stephen Appiah was also introduced in the second half.

One of the second half substitutes, Matthew Amoah made it 3-1 in 65 minutes.

But Japan produced a great come back in the last 13 minutes as Tamada, Okazaki and Junichi Inamoto scored to win the game for the Asian side.

Both sides are finalists for the 2010 World Cup having qualified from their respective zones.

The loss was the Black Stars first since they were beaten by Libya a year ago in the first phase of the 2010 World Cup qualifiers.