Wa All Stars' hopes of riding on the back of their fantastic Premier League victory against Hearts of Oak was cut short in the MTN FA Cup.
The Blues suffered a miserable second round exit and became the top-flight's first major causality in the Cup competition as they were trounced 3-0 by second tier side, Feyenoord Academy on Saturday in Fetteh.
The win showed Feyenoord's strong form this season as they are unbeaten in the Division One campaign with six wins and a draw in seven matches. They have now extended their unbeaten run to eight in all competitive matches.
However, the defeat exposed Wa All Stars' inconsistencies this season.
In a competition noted for producing upsets, Liberty Professionals, managed to avert an early exit with victory over Charity Stars.
After drawing 1-1 in regulation time, Liberty went on to win the game 4-3 on penalties as Charity Stars, who progressed to the Round of 64 after going through a marathon penalty shootout session of 40 kicks were unlucky this time.
Former Cup winners, Nania FC are through to the third round after defeating DC United 1-0 in Legon.
Red Lions FC recorded the biggest win with a 7-0 win against Binduri United.
Teams from the Premier League, Division One and Two are involved in the second round stage of the competition with matches running through the festive season and to December 29.
The team that emerges as winner of the MTN FA Cup competes in the CAF Confederations Cup.
Round of 64 Results
Matches played on December 21
Inter Allies 1-0 Berlin FC
Mighty Jets 3-1 Mepe United
Nania FC 1-0 DC United
Bubiashie Charity Stars 0-0(3-4) Liberty Professionals
Metro FC 4-0 Esperance
Future Stars 0-0(4-1) Bofoakwa
Sampa United 2-1 Bagojet
Tumu Happy Stars 0-1 Tema United
Red Lions FC 7-0 Binduri United
Feyenoord Academy 3-0 Wa All Stars